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  • Title: Efficient purification of somatomedin-C/insulin-like growth factor I using immunoaffinity chromatography.
    Author: Chernausek SD, Chatelain PG, Svoboda ME, Underwood LE, Van Wyk JJ.
    Journal: Biochem Biophys Res Commun; 1985 Jan 16; 126(1):282-8. PubMed ID: 3882085.
    Abstract:
    Somatomedin-C/insulin-like growth factor I was purified from human plasma using a monoclonal antibody affinity column. Combining immunoaffinity chromatography with standard protein purification methods resulted in an overall recovery of 18%. The 35 micrograms of somatomedin-C/insulin-like growth factor I purified from 500 ml of plasma appeared as a single band when analyzed by polyacrylamide gel electrophoresis and could be used in radioimmunoassay and receptor binding studies.
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