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  • Title: Escherichia coli mutants possessing an Li+-resistant melibiose carrier.
    Author: Shiota S, Yamane Y, Futai M, Tsuchiya T.
    Journal: J Bacteriol; 1985 Apr; 162(1):106-9. PubMed ID: 3884586.
    Abstract:
    Escherichia coli K-12 strains in the absence of the lactose carrier grew on the disaccharide melibiose as the sole source of carbon. The presence of 0.1 mM Li+ in the medium strongly inhibited growth of such cells, and Li+-resistant mutants appeared after several days of incubation. These mutants showed altered cation coupling to melibiose transport via the melibiose carrier. Cotransport between H+ and melibiose was lost in the mutants, although Na+-melibiose cotransport was retained. We observed no Li+-melibiose cotransport. Therefore, these mutants represent a new type of cation-coupling mutants of the melibiose carrier.
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