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  • Title: Mutations upstream of the ribosome-binding site affect translational efficiency.
    Author: Coleman J, Inouye M, Nakamura K.
    Journal: J Mol Biol; 1985 Jan 05; 181(1):139-43. PubMed ID: 3884820.
    Abstract:
    The DNA coding for the major outer membrane lipoprotein of Escherichia coli has been fused to the coding region of the beta-galactosidase gene to measure the effect of various mutations on the efficiency of translation initiation. The various mutants were made by either inserting or deleting a small number of nucleotides into or from a region just upstream of the ribosome-binding site. These small mutations dramatically affect translation initiation as measured by the production of beta-galactosidase. We postulate that these mutations affect translation initiation by altering the secondary structure of the messenger RNA. In one case, we predict that a stem and loop just upstream of the Shine-Dalgarno sequence sterically hinders the binding of the ribosome to the mRNA.
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