These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: An examination of the TRF assay reveals a heterogeneity of TRF-like activities.
    Author: Eisenberg L.
    Journal: J Immunol; 1985 Jun; 134(6):3920-5. PubMed ID: 3886792.
    Abstract:
    Supernatant from the cloned Mlsa,d-reactive helper T cell line L2V is a potent source of T cell replacing factor (TRF) activity. To determine whether L2V SF was representative of TRF-active supernatants, it was compared with supernatant from Con A-stimulated spleen cells (CASF) by using TRF assays. This analysis, facilitated by the use of four different antigens (R36a, TNP-R36a, SRBC, and HRBC), produced the following results. 1) L2V SF and CASF allowed responses of similar magnitude against either R36a or TNP-R36a; however, CASF always allowed responses of fivefold to 30-fold greater magnitude against SRBC than L2V SF. 2) B cells from xid and "normal" mice will give equally large responses to TNP-R36a when CASF is used as the source of TRF; however, L2V SF will only effect the responses by "normal" B cells. 3) L2V SF-driven responses to R36a and SRBC, and CASF-driven responses to R36a, follow single-hit kinetics and are IL 2 independent, whereas CASF-driven responses to SRBC follow multi-hit kinetics and are IL 2 dependent. These results indicate that the TRF assay measures a heterogeneity of TRF-like activities that can be distinguished according to the supernatant, antigen, and/or B cell responders used. The determination of whether this heterogeneity is due to the number of molecular components of a single "type" of TRF required for each antigen-induced PFC response or to the existence of distinct "types" of TRF is examined.
    [Abstract] [Full Text] [Related] [New Search]