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  • Title: Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome.
    Author: Mahmoud LM, Dutt M.
    Journal: Plant Cell Rep; 2024 Jun 14; 43(7):171. PubMed ID: 38874819.
    Abstract:
    A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.
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