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  • Title: Alternate radiolabeled markers for detecting metabolic activity of Mycobacterium leprae residing in murine macrophages.
    Author: Prasad HK, Hastings RC.
    Journal: J Clin Microbiol; 1985 May; 21(5):861-4. PubMed ID: 3889052.
    Abstract:
    This study demonstrated the utility of using 4% NaOH as a murine macrophage cell-solubilizing agent to discriminate between host macrophage metabolism and that of intracellular Mycobacterium leprae. A 4% concentration of NaOH had no deleterious effect on labeled mycobacteria. Thereby, alternate radiolabeled indicators of the metabolic activity of intracellular M. leprae could be experimented with. Significant incorporation of 14C-amino acid mixture, [14C]leucine, [14C]uridine, and carrier-free 32P was observed in cultures containing freshly extracted ("live") strains of M. leprae as compared with control cultures containing autoclaved bacilli.
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