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  • Title: Development of an indirect fluorescent antibody test, using microfluorometry as a diagnostic test for bovine anaplasmosis.
    Author: Goff WL, Johnson WC, Kuttler KL.
    Journal: Am J Vet Res; 1985 May; 46(5):1080-4. PubMed ID: 3890629.
    Abstract:
    The indirect fluorescent antibody test for the diagnosis of Anaplasma marginale infection in cattle was modified for use with microfluorometry. The test was standardized by use of a fluorometer that measures intensity of fluorescence. Standardization included A marginale-infected blood smears on microscope slides as antigen, serum from an inoculated calf as a positive control containing specific antibody, and an affinity-purified fluorescein-conjugated anti-bovine immunoglobulin as 2nd antibody. The modified test and microfluorometry allowed for titration of sera from A marginale (Florida isolate)-inoculated cattle with a degree of accuracy exceeding visual determinations. In addition, the fluorometric test was more sensitive than the complement fixation or card agglutination tests in identifying cattle that had previous Anaplasma infections.
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