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  • Title: TSAT: Efficient evaluation software for NGS data of phage/mirror-image phage display selections.
    Author: Altendorf T, Mohrlüder J, Willbold D.
    Journal: Biophys Rep (N Y); 2024 Sep 11; 4(3):100166. PubMed ID: 38909902.
    Abstract:
    Phage display and mirror-image phage display are commonly used techniques for the identification of binders that are specific to predefined targets. Recent studies demonstrated the effectiveness of next-generation sequencing (NGS) by increasing the amount of information extracted from selections. This allows for a better analysis and increases the possibility to select effective binders. A potential downside to NGS analysis of phage display selections is the increased workload that is needed to analyze the obtained information. Here, we report on the development of TSAT (target-specific analysis tool), software for user-friendly and efficient analysis of peptide sequence data from NGS of phage display selections.
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