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  • Title: Plasmodium falciparum: invasion and development in highly parasitized cultures.
    Author: Raventos-Suarez C.
    Journal: In Vitro Cell Dev Biol; 1985 Mar; 21(3 Pt 1):161-4. PubMed ID: 3891720.
    Abstract:
    We report that synchronized cultures of Plasmodium falciparum with up to 40% parasitized cells can be obtained with the use of low, red blood cell suspensions and only daily replacement of culture medium. These cultures contained not only a reduced proportion of uninfected red cells but also a population of cells with brief and equal time of exposure to culture conditions. Such high parasitemias are desirable for studies of ring-staged parasites (for which enrichment techniques are not available) and late-staged parasites when the manipulations for enrichment are inappropriate or unsuccessful.
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