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  • Title: Antibiotic monitoring in body fluids.
    Author: Rouan MC.
    Journal: J Chromatogr; 1985 May 10; 340():361-400. PubMed ID: 3894402.
    Abstract:
    Analytical procedures recently described for the quantitative determination of antibiotics in body fluids are reviewed. High-performance liquid chromatography (HPLC) and immunoassays appear as an alternative to current microbiological assays. HPLC has been applied to most antibiotics in clinical use and a major part of the review deals with this technique. Attention is given to sample pretreatment, characteristics of chromatography and detection, and limit of sensitivity. Non-isotopic immunoassays have been essentially applied to aminoglycosides and vancomycin and are also reviewed. Advantages and drawbacks of HPLC and immunoassays are presented.
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