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  • Title: The use of caged aeration for the growth of animal cells on microcarriers.
    Author: Whiteside JP, Farmer S, Spier RE.
    Journal: Dev Biol Stand; 1985; 60():283-90. PubMed ID: 3899790.
    Abstract:
    A simple, unaerated 0.1 litre microcarrier propagator was successfully sealed up to 1 litre using constant tip speed of the flexible wide blade stirrer. Further scale-up to 10 litres was not successful because of inadequate aeration. When this deficiency was corrected, using a sintered aerator, cell growth did not improve due to excessive foam production. This was overcome by carrying out the necessary aeration inside a special cage, located inside the propagator, which retained the bubbles and excluded the microcarriers. Using this device cell growth improved to levels similar to those obtained at the 0.1 and 1 litre scales.
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