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  • Title: Cells which contain S-100 protein in Hodgkin's disease: a quantitative study.
    Author: Sangster G, Crocker J, Jenkins R, Leyland MJ.
    Journal: J Clin Pathol; 1985 Sep; 38(9):1021-4. PubMed ID: 3900142.
    Abstract:
    Paraffin sections from a series of 50 lymph nodes affected by Hodgkin's disease were examined by means of the unlabelled primary antibody peroxidase-antiperoxidase method to detect those cells which contained S-100 protein. In addition, 15 lymph nodes showing reactive follicular hyperplasia were studied. A simple enumeration procedure (eyepiece graticule) was used to count the number of such cells in 20 standard 25 X microscope fields. In the specimens of nodular sclerosing Hodgkin's disease many cells positive for S-100 protein were present, in contrast to the other Rye subtypes, which showed a relative paucity. By comparison, the lymph nodes showing reactive follicular hyperplasia contained a similar number of cells containing S-100 to those seen in the nodular sclerosing lymph nodes affected by Hodgkin's disease.
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