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  • Title: Identification of proline carrier in Escherichia coli K-12.
    Author: Hanada K, Yamato I, Anraku Y.
    Journal: FEBS Lett; 1985 Oct 28; 191(2):278-82. PubMed ID: 3902503.
    Abstract:
    Proline carrier, a product of the putP gene of Escherichia coli, was identified as a 35 kDa cytoplasmic membrane protein by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Its identification was based on the following evidence: First, the density of the band corresponding to a 35 kDa protein correlated with the proline-binding activity of cytoplasmic membranes from putP-deficient and putP-amplified strains. Second, by the differential labeling method, the 35 kDa protein was specifically labeled with radioactive N-ethyl-maleimide. The 35 kDa protein was found to aggregate on heat treatment and to show abnormal mobility on SDS-PAGE.
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