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  • Title: Microcalorimetry studies of energy changes during the growth of Klebsiella aerogenes in simple salts/glucose media; correlation of specific power and size of the ATP pool.
    Author: Bowden CP, James AM.
    Journal: Microbios; 1985; 43(173):93-105. PubMed ID: 3903440.
    Abstract:
    A parallel study has been made of the variation of the ATP pool and the specific power of cells of Klebsiella aerogenes during aerobic growth in glucose-limited medium under carefully defined conditions of growth and test. During the early part of exponential growth there was a marked increase in both the ATP pool and the specific power to near constant values during the later stages of growth; oscillations about the mean values were observed for each parameter. With the exhaustion of glucose and the cessation of growth both and ATP pool and the specific power decreased rapidly, the ATP pool to a low constant value and the specific power to zero. Changes in the values of these parameters during growth are discussed and it is concluded that the specific power is more dependent on the rate of catabolism rather than on the degree of coupling while the opposite is true for the ATP pool. Both parameters are, however, indicators of similar metabolic processes.
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