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  • Title: [Hormonal response following the use of gonadotropin-releasing hormone, estradiol and gestil in sheep].
    Author: Batzhargalin E.
    Journal: Vet Med Nauki; 1985; 22(9):60-6. PubMed ID: 3909630.
    Abstract:
    Studies were carried out in an anestral season with three ovariectomized and four intact sheep to establish the function of the hypothalamic-pituitary-ovarian axis. It was found that following three-fold injections with GnRH (250 ng, 250 ng, and 40 micrograms) at 2-hour intervals an immediate rise of the luteinizing hormone in the peripheral blood followed, tending to increase further with each injection. The concentration of this hormone after treatment with 17-beta-estradiol at the rate of 20 micrograms was first suppressed below the initial level up to the 8-12th hour, followed by a peak in its release between the 12th and the 24th hour. At stimulation with gonadotropic preparations the ovaries in the intact sheep responded with the gradual rise of the 17-beta-estradiol level, reaching a maximum at the 18th hour following injection, while in the ovariectomized animals there were no essential changes with the treatment during the experimental period. Data made it reasonable to believe that the application of these preparations might be useful in testing the functional state of the hypothalamic-pituitary-ovarian axis in sheep.
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