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  • Title: Protease susceptibility of zinc- and apo-carboxypeptidase A.
    Author: Bicknell R, Schaeffer A, Auld DS, Riordan JF, Monnanni R, Bertini I.
    Journal: Biochem Biophys Res Commun; 1985 Dec 17; 133(2):787-93. PubMed ID: 3910041.
    Abstract:
    Proteases in preparations of carboxypeptidase A progressively inactivate solutions of the apoenzyme but not the metal-containing enzyme. Free amino acids generated by proteolysis interfere with spectral studies after reconstituting the apoenzyme with cobalt. Purification by affinity chromatography eliminates this effect. Affinity-purified apoenzyme is susceptible to digestion with chymotrypsin but the metalloenzyme is not.
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