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  • Title: Monoclonal antibodies against MHC class II antigens elicited with a human non-T, non-B acute lymphoblastic leukemia cell line.
    Author: Poláková K, Chorváth B, Sedlák J, Duraj J, Matoska J, Karpatová M.
    Journal: Neoplasma; 1985; 32(6):641-8. PubMed ID: 3911080.
    Abstract:
    A series of seven monoclonal antibodies recognizing cell surface antigens of similar distribution on a panel of leukemia/lymphoma and lymphoblastoid human cell lines was prepared by hybridoma technique after immunization with non-T, non-B acute lymphoblastic leukemia cell line REH. Immune reactivity of these monoclonal antibodies with B-lymphoblastoid and lymphoma cell lines, as well as with non-T, non-B leukemia cell lines but not with T-leukemic and myeloid leukemic cell lines (with the exception of myeloid leukemia cell line KG 1) was demonstrated by indirect immunofluorescence. No reactivity was observed with the examined human non-hemopoietic tumor cell lines, except melanoma cell lines HMB-2 and SK 1477. These antibodies immunoprecipitated a similar cell surface bimolecular glycoprotein complex consisting of two glycosylated chains (gp30, 35), as demonstrated by immunoprecipitation of cell surface 125I-lactoperoxidase radioiodinated, periodate/tritiated borohydride radiolabeled and 35S-methionine metabolically radiolabeled proteins of REH cells. These properties, typical of MHC class II antigens correspond also to immunoperoxidase staining of lymph nodes with some selected antibodies.
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