These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: High-Pressure Freezing and Low-Temperature Processing of Seeds for Electron Microscopy and Electron Tomography.
    Author: Li J, Gou L, Shen J, Cao W.
    Journal: Methods Mol Biol; 2024; 2841():207-214. PubMed ID: 39115780.
    Abstract:
    High-pressure freezing/freeze substitution has been used to preserve biological samples for ultrastructure study instead of chemical fixation. For most plant samples, the water content is too high and cannot be properly preserved during cryofixation. Additionally, the cell wall is a barrier that prevents the substitution of water with the resin. In this chapter, we will discuss modified high-pressure freezing and subsequent processing protocols based on our routinely used methodology for examining Arabidopsis seeds in transmission electron microscopy and electron tomography.
    [Abstract] [Full Text] [Related] [New Search]