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  • Title: [Cloning of the hemagglutinin gene of influenza virus of subtype H3 in E. coli].
    Author: Rozinov MN, Gol'tsmaĭer TA, Dikarev SD, Gershanovich VN.
    Journal: Mol Gen Mikrobiol Virusol; 1985 Dec; (12):12-4. PubMed ID: 3916214.
    Abstract:
    dsDNA of the influenza virus subtype A/Leningrad/385/80/R (H3N2)-recombinant A/Leningrad/385/80 (H3N2) and RR/8/34 (H1N1) has been synthesized using polyadenylated viral RNA as a template. This dsDNA has been cloned on plasmid pUC19. A clone has been selected harbouring the plasmid with included proximal fragment of hemagglutinin gene that contains the main antigenic determinants. The hybrid plasmid is hybridizable with RNA of the hemagglutinin gene and with oligonucleotide CATGCAAAACCTTCCC that is complementing the sequence coding for the proximal fragment of the mature hemagglutinin.
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