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  • Title: beta-Glucosidase and beta-galactosidase in primary cultures of rat astrocytes: comparison to the brain enzymes.
    Author: Hof L, Kimelberg HK.
    Journal: J Neurochem; 1985 Jan; 44(1):274-7. PubMed ID: 3917292.
    Abstract:
    In primary astrocyte cultures beta-glucosidase (EC 3.2.1.21) and beta-galactosidase (EC 3.2.1.23) showed pH optima and Km values identical to rat brain enzymes, using methylumbelliferyl glycosides and labeled gluco- and galactocerebrosides as substrates. The activities of both glycosidases increased in culture up to 3-4 weeks. In rat brain only galactosidase increased; glucosidase activity declined between 12-20 days after birth. The specific activities were two- to sixfold higher in astrocyte cultures than in rat brain. These activities were not due to uptake of enzymes from the growth medium. Secretion of beta-galactosidase, but not beta-glucosidase nor acid phosphatase could be demonstrated. These results support the suggestion of a degradative function for astrocytes in the brain.
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