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  • Title: [Effect of the fetal testis on the number of germ cells in the fetal ovary of rats in vitro].
    Author: Prepin J, Charpentier G, Jost A.
    Journal: C R Acad Sci III; 1985; 300(2):43-7. PubMed ID: 3918775.
    Abstract:
    When ovaries from 13.5-day-old fetuses are explained and cultured in vitro for 4 days in a synthetic medium, the number of germ cells increases 6 fold, on average. This increase is only approximately 2 fold if a pair of 16.5-day fetal testes is cultured together with the ovaries or if the ovaries are cultured in a medium in which testes have previously been grown for 4 days. The effect of the latter medium persists if it is dialysed against fresh medium, which suggests that the conditioned medium contains one or several substance(s) of molecular weight superior to the cut-off of the membrane. The testicular effect seems to be effective mainly during the final phase of intense multiplication of the germ cells.
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