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  • Title: Measurement of xylanase activity with insoluble xylan substrate.
    Author: Nummi M, Perrin JM, Niku-Paavola ML, Enari TM.
    Journal: Biochem J; 1985 Mar 01; 226(2):617-20. PubMed ID: 3922355.
    Abstract:
    Insoluble xylan was prepared from ground birch (Betula pubescens) pulp by alkali extraction and precipitation with ethanol. The only sugar detected after acid hydrolysis of the preparation was xylose. The insoluble xylan was used as substrate in a nephelometric assay to determine the xylanase (EC 3.2.1.8, 1,4-beta-D-xylan xylanohydrolase and EC 3.2.1.37, 1,4-beta-D-xylan xylohydrolase) activities of Aspergillus and Trichoderma enzymes. The nephelometric method is reliable in evaluating xylanase hydrolysis of insoluble xylan.
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