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  • Title: Stable hyper-production of Escherichia coli beta-lactamase by Bacillus subtilis grown on a 0.5 M succinate-medium using a B. subtilis alpha-amylase secretion vector.
    Author: Nakamura K, Furusato T, Shiroza T, Yamane K.
    Journal: Biochem Biophys Res Commun; 1985 Apr 30; 128(2):601-6. PubMed ID: 3922363.
    Abstract:
    Extracellular production of Escherichia coli beta-lactamase by Bacillus subtilis, using a B. subtilis secretion vector constructed from its own alpha-amylase gene, was promoted when the cells were grown on LG-medium containing 0.5 M succinate under poor aeration conditions. The amount of the enzyme secreted was 50 to 60 times as large as that obtained by cultivation of the cells on LG-medium under good aeration conditions. The effect of protease-deficient mutant of B. subtilis on the enzyme production by B. subtilis was significant while that of protease inhibitors was negligible or inexistent.
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