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  • Title: Two techniques for detection of antibodies against Corynebacterium (Rhodococcus) equi in horse sera.
    Author: Skalka B, Svastová A.
    Journal: Vet Microbiol; 1985 Apr; 10(3):293-300. PubMed ID: 3923693.
    Abstract:
    Two techniques were developed to detect antibodies against the exosubstance of C. equi called equi-factor. In the first technique serum samples are tested against native equi-factor produced by the growth of C. equi on agar medium. A positive result is manifested by the development of precipitation lines. The second test is based on neutralization of prepurified equi-factor by antibody, resulting in the inhibition of its hemolytic synergism with staphylococcal beta toxin. Sera (125 samples) from horses of different ages, kept in localities with a history of C. equi infections, were examined. The first technique detected 65.6%, and the second 40% of positive cases.
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