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  • Title: Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2.
    Author: Golden SS, Haselkorn R.
    Journal: Science; 1985 Sep 13; 229(4718):1104-7. PubMed ID: 3929379.
    Abstract:
    A psbA gene encoding the target of photosystem II herbicide inhibition, the 32,000-dalton thylakoid membrane protein, has been cloned from a mutant of Anacystis nidulans R2, which is resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea-(diuron). A cloned DNA fragment from within the coding region of this gene transforms wild-type cells to herbicide resistance, proving that mutation within psbA is responsible for that phenotype. The mutation consists of a single nucleotide change that replaces serine at position 264 of the wild-type protein with alanine in that of the diuron-resistant mutant.
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