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  • Title: Antibody determination against Aspergillus fumigatus by means of the enzyme-linked immunosorbent assay. II. Physico- and immunochemical properties of the polystyrene-binding components.
    Author: Kauffman HF, van der Heyden PJ, van der Laan S, van der Heide S, Beaumont F, de Vries K.
    Journal: Int Arch Allergy Appl Immunol; 1985; 78(2):174-81. PubMed ID: 3930408.
    Abstract:
    Polystyrene-binding antigenic components (PBC) of Aspergillus fumigatus involved in ELISA IgG antibody determinations and the corresponding numbers of precipitates found by double immunodiffusion (DID) and/or crossed immunoelectrophoresis (CIE) were studied using culture filtrate antigenic preparations fractionated by Sephadex G-100 chromatography, concanavalin A (ConA) affinity chromatography, isoelectric focusing (IEF) and TCA precipitation. It is shown that PBC, immunologically active in ELISA, are characterized by high molecular weights and ConA-binding properties. The pI values of these components were 3.5-6 and above 8. The glycoprotein and/or mucopolysaccharide nature is suggested by the binding to ConA and their partial resistance to trichloroacetic acid treatment. By contrast, low-molecular weight components and antigenic components that are not absorbed by ConA being active in DID and/or CIE showed less or absence of binding to the polystyrene surface.
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