These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Genetic polymorphisms of common sandflies in selected areas of Henan Province based on DNA barcoding].
    Author: Wang D, He Z, Yang C, Lu D, Sun Y, Kou Y, Qian D, Zhang H, Liu Y.
    Journal: Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi; 2024 Aug 05; 36(4):352-360. PubMed ID: 39322294.
    Abstract:
    OBJECTIVE: To characterize the species of common sandflies in Henan Province using DNA barcoding with cytochrome c oxidase subunit I (COI) gene as the molecular marker, and to analyze the genetic polymorphisms of sandflies, so as to provide insights into visceral leishmaniasis prevention and control in Henan Province. METHODS: Sandfly specimens were sampled from 13 sandflies surveillance sites from 2021 to 2023 in Anyang City, Zhengzhou, Luoyang and Xuchang cities (Zhengzhou-Luoyang-Xuchang areas) where visceral leishmaniasis cases were reported and in Jiaozuo and Xinxiang cities (Jiaozuo-Xinxiang areas) without visceral leishmaniasis cases reported. Genomic DNA was extracted from a single sandfly, and COI gene was amplified. The amplification product was subjected to bidirectional sequencing. Following sequence assembly, the species of sandflies was characterized through sequence alignment using the BLAST tool. The intra-specific and inter-specific genetic distances of sandflies were estimated among different areas using the software Mega 11, and phylogenetic trees were created. The polymorphisms of nucleotide sequences in the sandflies COI gene were estimated using the software DnaSP. The fixation index (FST) of different geographical isolates of sandflies was calculated using the Arlequin software, and the gene flow value (Nm) was used to measure the gene flow in the sandflies populations. In addition, the population genetic structure of different geographical populations of Phlebotomus chinensis was analyzed using the STRUCTURE software. RESULTS: A total of 978 sandflies were collected from 13 sandflies surveillance sites in Zhengzhou-Luoyang-Xuchang areas, Jiaozuo-Xinxiang areas and Anyang City of Henan Province from 2021 to 2023, and 475 sandflies were randomly sampled for subsequent detections. A total of 304 Ph. chinensis, 162 Se. squamirostris and 9 Se. bailyi were identified based on molecular biological detection of the COI gene, and Se. bailyi was reported for the first time in Henan Province. The intraspecific genetic distances of sandflies were 0.000 to 0.040, and the inter-specific genetic distances ranged from 0.133 to 0.161. Phylogenetic analysis revealed that each of the three sandfly species was clustered into a clade. The genetic polymorphisms of Ph. chinensis populations varied among different areas, with the highest haplotype diversity (0.966 ± 0.007) and the greatest nucleotide diversity (0.011) in Zhengzhou-Luoyang-Xuchang areas, and the lowest haplotype diversity (0.720 ± 0.091) and nucleotide diversity (0.004) in Anyang City. The dominant haplotype of Ph. chinensis populations was Pch_Hap_2 in Anyang City and Jiaozuo-Xinxiang areas, with moderate genetic differentiation (0.05 < FST < 0.15) and frequent gene exchange (Nm value > 1) between Ph. chinensis populations sampled from Anyang City, and Jiaozuo-Xinxiang areas. Population genetic structure analysis showed that the dominant component of Ph. chinensis populations was K5 in Anyang City and Jiaozuo-Xinxiang areas. No obvious dominant haplotype was observed in Ph. chinensis populations sampled from Zhengzhou-Luoyang-Xuchang areas, which had very high genetic differentiation (FST > 0.25) and little gene exchange (Nm value < 1) with Ph. chinensis populations from Anyang City, and Jiaozuo-Xinxiang areas, with K3 as the dominant component. In addition, there was no significant difference in the genetic polymorphism level among Se. squamirostris populations from the three areas. CONCLUSIONS: There are Ph. chinensis, Se. squamirostris and Se. bailyi in Henan Province, and S. bailyi is recorded for the first time in Henan Province by molecular biological assays. There are different levels of genetic differentiation and gene exchange among P. chinensis populations in different areas of Henan Province. ［摘要］ 目的 应用以细胞色素c氧化酶亚基 (I cytochrome c oxidase subunit I, COI) 基因为分子标记的DNA条形码技术 鉴定河南省常见白蛉蛉种, 分析其种群遗传多态性, 为该省内脏利什曼病防控提供科学依据。方法 2021—2023年, 在 河南省有内脏利什曼病病例报告的安阳片区, 郑州、洛阳、许昌 (郑洛许) 片区和无病例报告的焦作、新乡 (焦新) 片区的 13个白蛉监测点采集白蛉样本, 提取单只白蛉基因组DNA并扩增其COI 基因。对扩增产物进行双向测序, 拼接序列经 BLAST比对鉴定蛉种。采用Mega 11软件计算不同片区白蛉种内与种间遗传距离, 并构建系统发育树。采用DnaSP软件 计算白蛉COI 基因核苷酸序列多态性, 采用Arlequin软件计算不同地理种群间的遗传分化指数 (FST), 采用基因流值 (Nm) 衡量群体中基因流动, 采用STRUCTURE软件对中华白蛉不同地理种群进行群体遗传结构分析。结果 2021— 2023年, 自河南省郑洛许片区、焦新片区以及安阳片区13个白蛉监测点累计采集978只白蛉, 随机选择其中475只进行 检测。基于COI 基因的分子生物学检测鉴定出中华白蛉304只、鳞喙司蛉162只、贝氏司蛉9只, 其中贝氏司蛉为河南省 首次发现。各蛉种种内遗传距离为0.000 ~ 0.040, 种间遗传距离为0.133 ~ 0.161。系统发育树分析显示, 3种蛉种各自形 成一个进化支。各片区中华白蛉种群遗传多态性水平存在较大差异, 其中郑洛许片区单倍型多样性 (0.966 ± 0.007) 和核酸 多样性 (0.011) 均最高, 安阳片区单倍型多样性 (0.720 ± 0.091) 和核酸多样性 (0.004) 均最低。安阳片区、焦新片区中华白蛉 种群优势单倍型均为Pch_Hap_2, 2个片区种群间存在中度遗传分化 (0.05 < FST < 0.15) 和较频繁的基因交流 (Nm 值> 1)。种群遗传结构分析显示, 安阳片区、焦新片区中华白蛉种群优势组分均为K5。郑洛许片区中华白蛉种群无明显优势单 倍型, 与另2个片区中华白蛉种群之间均存在极高遗传分化 (FST > 0.25) 和极少基因交流 (Nm 值< 1), 其种群优势组分为 K3。3个片区鳞喙司蛉种群遗传多态性水平差异不大。结论 河南省存在中华白蛉、鳞喙司蛉、贝氏司蛉3种白蛉, 其中 贝氏司蛉为该省首次通过分子生物学方法鉴定发现。省内不同片区中华白蛉种群存在不同程度的遗传分化和基因 交流。.
    [Abstract] [Full Text] [Related] [New Search]