These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Determination and antioxidant analysis of seven flavonoids in bamboo-leaf extracts].
    Author: Gu LL, Yao X, An RM, Guo XF.
    Journal: Se Pu; 2024 Oct; 42(10):972-978. PubMed ID: 39327661.
    Abstract:
    The flavonoid contents of different bamboo-leaf extracts and their relationships to antioxidant activity were investigated in this study by preparing nine samples using two commercially available bamboo-leaf extract products and seven bamboo-leaf extracts such as Phyllostachys edulis. A high performance liquid chromatography (HPLC) method was established to determine seven flavonoid components (orientin, isoorientin, vitexin, isovitexin, tricin, luteolin and luteoloside) in these samples, which were separated using a SymmetryShieldTM RP8 column (250 mm×4.6 mm, 5 μm) under gradient-elution conditions using acetonitrile as mobile phase A and 0.5% (v/v) acetic acid aqueous solution as mobile phase B. The antioxidant activities of the samples were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical-scavenging assays, with half inhibitory concentration (IC50) as an indicator and the butylated hydroxytoluene (BHT) and tert-butylhydroquinone (TBHQ) antioxidants as positive controls. Pearson correlation was then used to analyze the relationship between flavonoid content and antioxidant activity. The HPLC method was found to be accurate and reliable for determining the flavonoid contents of the bamboo-leaf extracts. The seven flavonoids were well separated, and good linear relationships were exhibited (correlation coefficients (R2)≥0.9990). Furthermore, the contents of the seven flavonoids in the bamboo-leaf extracts ranged from 14.97 to 183.94 mg/g, with the highest content of 183.94 mg/g recorded for Phyllostachys edulis. The bamboo species exhibited significantly different flavonoid contents, with Phyllostachys edulis showing the highest orientin, isoorientin, and vitexin levels of 38.45, 101.30, and 9.42 mg/g, respectively. Moreover, the bamboo-leaf extracts exhibited IC50 values of 78.23-179.41 mg/L for DPPH-radical-scavenging, while values of 203.48-1250.81 mg/L were recorded for hydroxyl radicals. The Phyllostachys edulis leaf extract exhibited the strongest antioxidant activity, with the lowest IC50 values of 78.23 and 203.48 mg/L for DPPH and hydroxyl, respectively; it showed greatly significant for the further development and application of Phyllostachys edulis. Finally, the relationships between flavonoid content and the DPPH- and hydroxyl-radical-scavenging activities (based on the IC50 values) were correlated, which revealed that the orientin and isoorientin contents are closely related to the antioxidant activities of the bamboo-leaf extracts. Consequently, the orientin and isoorientin contents can be used as indicators for evaluating the antioxidant activities of bamboo-leaf extracts. 为研究不同竹叶提取物中黄酮成分含量及其与抗氧化活性之间的关系,以2种市售竹叶提取物以及以毛竹等7种竹种的竹叶为材料制备的竹叶提取物共9个样品为研究对象,建立了同时测定竹叶提取物中7种黄酮成分(荭草苷、异荭草苷、牡荆苷、异牡荆苷、苜蓿素、木犀草素和木犀草苷)的高效液相色谱(HPLC)方法,采用SymmetryShieldTM RP8色谱柱(250 mm×4.6 mm, 5 μm),以乙腈为流动相A, 0.5%(v/v)乙酸水溶液为流动相B进行梯度洗脱。采用1,1-二苯基-2-三硝基苯肼(DPPH)和羟基自由基清除试验评价竹叶提取物的抗氧化活性,以半抑制浓度(IC50)为评价指标,以抗氧化剂2,6-二叔丁基对甲酚(BHT)和叔丁基对苯二酚(TBHQ)为阳性对照,并利用Person相关性分析各黄酮成分含量与抗氧化的关系。结果表明:建立的HPLC测定方法准确可靠,适用于竹叶提取物中黄酮含量的测定。竹叶提取物中7种黄酮含量在14.97~183.94 mg/g范围内,毛竹中7种黄酮总含量最高,为183.94 mg/g。不同竹种间7种黄酮成分的含量存在显著差异,荭草苷、异荭草苷和牡荆苷含量均在毛竹中最高,分别为38.45、101.30和9.42 mg/g。竹叶提取物清除DPPH自由基的IC50值在78.23~179.41 mg/L范围内,清除羟基自由基的IC50值在203.48~1250.81 mg/L范围内。毛竹叶提取物清除DPPH和羟基自由基的IC50值分别为78.23 mg/L和203.48 mg/L,其抗氧化活性最强,具有开发应用价值。相关性分析结果表明,荭草苷、异荭草苷含量与竹叶提取物抗氧化活性密切相关。
    [Abstract] [Full Text] [Related] [New Search]