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  • Title: Purification of peroxisomes from livers of normal and clofibrate-treated mice.
    Author: Crane DI, Hemsley AC, Masters CJ.
    Journal: Anal Biochem; 1985 Aug 01; 148(2):436-45. PubMed ID: 3933379.
    Abstract:
    A method for the isolation of peroxisomes from livers of normal and clofibrate-treated mice is described. The method utilizes glutaraldehyde to stabilize peroxisomal membranes, and isopycnic centrifugation of a light mitochondrial fraction through a linear metrizamide gradient to achieve optimal resolution from other organelles. On the basis of the biochemical and morphological data, the peroxisomal preparations are indicated as of high purity: contamination by mitochondria, lysosomes, and plasma membranes is negligible, and the level of contaminating microsomes is around 5% for normal peroxisomes and 8% for peroxisomes from clofibrate-treated mice. Peroxisomal membranes prepared by carbonate extraction contain two major polypeptides of approximately 70,000 Da, and show 2 and 8% contamination by microsomal membrane protein for the preparations from normal and clofibrate-treated mice, respectively.
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