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  • Title: Ascorbate induced lipid peroxidation results in loss of receptor binding in tris, but not in phosphate, buffer. Implications for the involvement of metal ions.
    Author: Abe K, Kogure K, Arai H, Nakano M.
    Journal: Biochem Int; 1985 Sep; 11(3):341-8. PubMed ID: 3933512.
    Abstract:
    Rat brain homogenate was incubated with various concentrations of ascorbate in a Tris-HC1 buffer. Thiobarbituric acid-reactive substances (TBARS) were measured and 3H-QNB (quinuclidinyl benzilate) binding was also assayed on the same homogenate. Good parallelism between TBARS formation and loss of 3H-QNB binding activity confirmed that loss of 3H-QNB binding resulted from ascorbate-induced lipid peroxidation. However, neither formation of TBARS nor loss of 3H-QNB binding occurred in phosphate buffer or in the Tris-HC1 system in the presence of metal-chelating reagents. This indicates that phosphate addition prevents the ascorbate effects due to complete chelation of intrinsic metal ions.
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