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  • Title: A HOF-101@AgNPs-based dual-signal mode aptasensor for electrochemiluminescence and fluorescence detection of E. coli O157:H7.
    Author: Yang L, Wang X, Jing X, Bai B, Bo T, Zhang J, Yu L, Qian H, Gu Y, Yang Y.
    Journal: Food Chem; 2025 Feb 01; 464(Pt 1):141591. PubMed ID: 39396474.
    Abstract:
    Escherichia coli O157:H7 (E. coli O157:H7) emerges as a foodborne pathogen, emphasizing the imperative for creating precise detection tools. An ultrasensitive electrochemiluminescence/fluorescence (ECL/FL) dual-signal mode aptasensor was constructed for the detection of E. coli O157:H7. Ultrafine silver nanoparticles were loaded on the surface of hydrogen-bonded organic skeleton materials by in-situ photoreduction method, and then combined with aptamers that can identify specific targets to prepare HOF-101@AgNPs@Apt, greatly simplifying the ECL/FL dual-signal mode probe fabrication process and improving sensing reliability. HOF-101@AgNPs@Apt had both strong ECL luminescence and fluorescence, resulting in a high detection sensitivity. The low limit of detection (LOD) for ECL and FL were 0.48 CFU mL-1 and 2.39 CFU mL-1, respectively. Moreover, the proposed dual-signal mode aptasensor has been successfully applied to determine E. coli O157:H7 levels in tap water and milk with superior accuracy and high antiinterference capability, providing a promising method for food safety monitoring.
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