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  • Title: Selective extraction of the DM-20 brain proteolipid.
    Author: Skalidis G, Trifilieff E, Luu B.
    Journal: J Neurochem; 1986 Jan; 46(1):297-9. PubMed ID: 3940288.
    Abstract:
    Brain DM-20 proteolipid was previously shown to be structurally different from the myelin major proteolipid (MMPL). In an attempt to set up a large-scale purification of DM-20, we studied extraction of brain proteolipids with mixtures of methylene chloride containing up to 80% methanol. The CH2Cl2-CH3OH (3:7, vol/vol) mixture is highly selective for the extraction of bovine brain DM-20 compared to MMPL. Purified DM-20 was obtained after chromatography of the extract on methylated Sephadex.
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