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  • Title: Asymmetrical flow field-flow fractionation coupled with multi-detector: A robust method for evaluating the degradation of jujube polysaccharides with ultrasound-assisted H2O2 and subsequent conjugation with soybean 7S globulin.
    Author: Li J, Zhang X, Lian H, Zhang C, Zheng H, Han D, Dou H.
    Journal: Food Chem; 2025 Feb 01; 464(Pt 1):141609. PubMed ID: 39406136.
    Abstract:
    Soybean 7S globulin (7S) is a main allergen in soybean. In this study, 7S was modified with jujube polysaccharide (JP) via the Maillard reaction. The effects of ultrasound-assisted hydrogen peroxide (US/H2O2) treatment on the degradation degree of JP were investigated by size exclusion chromatography (SEC) and asymmetrical flow field-flow fractionation (AF4) coupled online with ultraviolet-visible (UV), multiangle light scattering (MALS), and differential refractive index (dRI) detectors. The effects of the degradation degree of JP on the antigenicity of 7S were investigated by a direct competitive ELISA method. The results demonstrated that the degradation degree of JP treated with 2.0 % H2O2 was the largest, which promoted the extent of the Maillard reaction with 7S, and eventually formed a compact JP-7S conjugate, which might be conducive to reducing the antigenicity of 7S. AF4-UV-MALS-dRI proved to be a useful method for evaluating the degradation degree of JP and the formation of JP-7S conjugates.
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