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  • Title: A spectrophotometric assay for the characterization of the S' subsite specificity of alpha-chymotrypsin.
    Author: Schellenberger V, Jakubke HD.
    Journal: Biochim Biophys Acta; 1986 Jan 17; 869(1):54-60. PubMed ID: 3942751.
    Abstract:
    The partitioning of maleylphenylalanine-alpha-chymotrypsin formed using maleylphenylalanine methyl ester as acyl donor between amino acid-derived nucleophiles and water was determined spectrophotometrically. The interpretation of the results obtained from graphical analysis gave evidence of a high conformational specificity of alpha-chymotrypsin towards basic amino acid derivatives in the P'1 position including significant differences between the amide and methyl ester of arginine. Amides of neutral amino acids with large side-chains show higher nucleophile reactivity in comparison with glycine amide.
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