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  • Title: Use of Staphylococcus aureus for rapid radioimmunoassay of influenza A virus haemagglutinin.
    Author: Poláková K, Simkovicová M, Chorváth B, Russ G, Styk B, Sourek J.
    Journal: Acta Virol; 1979 Mar; 23(2):107-12. PubMed ID: 39433.
    Abstract:
    In a rapid method for the radioimmunoassay (RIA) of influenza A virus haemagglutinin, Staphylococcus aureus (strain Cowan I, Czechoslovak State Collection No Mau 55/64) was used for separation of bound and free antigens. With rabbit and human immune sera, the binding of antigen-antibody complexes to heat-killed, formalin-fixed staphylocci was comparable to the double antibody technique. The time required for the completion of binding reaction was about 10 min compared to 18--24 hr required for double antibody precipitation. S. aureus did not bind directly (i.e. in the absence of specific antibody) a significant amount of radiolabelled antigen.
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