These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Change of inverted thyroid follicle into a spheroid after embedding in a collagen gel.
    Author: Garbi C, Tacchetti C, Wollman SH.
    Journal: Exp Cell Res; 1986 Mar; 163(1):63-77. PubMed ID: 3943564.
    Abstract:
    When inverted thyroid follicles in suspension culture are embedded in a collagen gel, there is extensive reorganization of the follicle. To identify intermediate stages in the reorganization, a suspension of inverted follicles was mixed with a cold solution of collagen (0.1 mg/ml) in culture medium and the resultant was warmed and allowed to gel. Prior to embedding, the epithelial cells bounding the lumens formed a monolayer of attenuated cells with their microvilli-bearing surface in contact with the medium. The first change noted was a shrinkage of the lumen in many follicles by 18 h. The cells became cuboidal to columnar. Some of the cells had long sheet-like processes extending into the lumen in contact with those of other cells. In late stages of the reorganization, 48 h, the cells were arranged in a compact spheroid. The spheroids contained two different kinds of colloid-filled lumens, possibly of different origins, one a spherical microlumen, the other very long and narrow in section. The peripheral cells of the spheroid had a smooth plasma membrane (without microvilli) in contact with collagen. Although most of the cells in a section had a microvilli-bearing surface forming part of the boundary of a lumen, it is not certain that all cells were in contact with a lumen.
    [Abstract] [Full Text] [Related] [New Search]