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  • Title: Postreplication repair in ultraviolet-irradiated human fibroblasts: formation and repair of DNA double-strand breaks.
    Author: Wang TC, Smith KC.
    Journal: Carcinogenesis; 1986 Mar; 7(3):389-92. PubMed ID: 3948324.
    Abstract:
    A neutral filter elution assay was used to determine if the postreplicational formation and repair of DNA double-strand breaks (DSB) occurs in u.v.-irradiated human cells. Excision-deficient XP12 cells were pulse-labeled with [3H]thymidine after u.v. irradiation (1.5-3 J/m2), and the nascent DNA was followed during repair incubation. With increasing u.v. radiation fluences, an increasing fraction of DNA was eluted at a fast rate, indicating that DSB were produced. The maximum yield of DSB was observed after about 24 h of postirradiation incubation at 37 degrees C. Similar results were also obtained with repair-proficient VA13 cells when irradiated at much higher fluences (7.5-15 J/m2). It is concluded that, at the u.v. radiation fluences used in this work, the DSB produced in u.v.-irradiated human cells are the result of post-replication repair events, and at incubation times greater than 24 h some of these DSB are repaired.
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