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  • Title: Vasopressin-stimulated phosphorylation of rat liver phospholipid methyltransferase in isolated hepatocytes.
    Author: Mérida I, Varela I, Alvarez JF, Cabrero C, Mato JM.
    Journal: FEBS Lett; 1986 Feb 17; 196(2):274-8. PubMed ID: 3949001.
    Abstract:
    Addition of vasopressin (1 microM) to isolated rat hepatocytes prelabeled with [32P]phosphate was accompanied by a 250% increase in the phosphorylation of phospholipid methyltransferase. Vasopressin-stimulated phospholipid methyltransferase phosphorylation was time- and dose-dependent. 32P-labeled phospholipid methyltransferase was recovered by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. After electrophoresis, phospholipid methyltransferase was electroeluted from the polyacrylamide gel and subjected to tryptic digestion or HCl hydrolysis. Analysis of 32P-labeled peptides reveals only one site of phosphorylation and the analysis of [32P]phosphoamino acids indicates that phosphoserine is the only labeled amino acid.
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