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  • Title: Thin layer chromatographic method for determination of deoxynivalenol in wheat: collaborative study.
    Author: Eppley RM, Trucksess MW, Nesheim S, Thorpe CW, Pohland AE.
    Journal: J Assoc Off Anal Chem; 1986; 69(1):37-40. PubMed ID: 3949697.
    Abstract:
    A collaborative study of a rapid method for the determination of deoxynivalenol (DON) in winter wheat was successfully completed. The method involves sample extraction with acetonitrile-water (84 + 16), cleanup using a disposable column of charcoal, Celite, and alumina, and detection by thin layer chromatography after spraying with an aluminum chloride solution. Each of the 15 collaborators analyzed 12 samples, 2 of which were naturally contaminated, and 10 to which DON was added, in duplicate, at levels of 0, 50, 100, 300, and 1000 ng/g. Average recoveries of DON ranged from 78 to 96% with repeatabilities of 30-64% and reproducibilities of 33-87%. The results of the study show that false positives were not a problem and that all of the analysts could detect DON at the 300 ng/g level or higher. The method has been adopted official first action.
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