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  • Title: Ca++ localization in boar spermatozoa by the pyroantimonate technique and X-ray microanalysis.
    Author: Berruti G, Franchi E, Camatini M.
    Journal: J Exp Zool; 1986 Feb; 237(2):257-62. PubMed ID: 3950567.
    Abstract:
    Intracellular, loosely bound Ca++ has been localized electron microscopically in freshly ejaculated boar spermatozoa by in situ precipitation with potassium antimonate. Ca++ was identified as the cation precipitated by testing the EGTA-sensitivity of the precipitates and by X-ray microprobe analysis. The data obtained revealed that the outer acrosomal membrane is the preferential site for Ca++ precipitation in the sperm head.
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