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  • Title: Temperature-dependent cleavage of chromatin by micrococcal nuclease near the nucleosome center.
    Author: Huang SY, Garrard WT.
    Journal: FEBS Lett; 1986 Apr 07; 199(1):89-91. PubMed ID: 3956749.
    Abstract:
    Digestion of nuclei at 4 degrees C with micrococcal nuclease results in significant intranucleosomal cleavage compared to digestion conducted at 37 degrees C. Employing nucleoprotein gel electrophoresis in one dimension followed by DNA electrophoresis in a second dimension, we demonstrate that such temperature-sensitive, internal cleavage predominantly occurs about 20 bp from the nucleosome center. We suggest that lower temperatures reduce the stability of hydrophobic interactions within the histone octamer and lead to a conformational alteration in nucleosomes that is detected by micrococcal nuclease.
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