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  • Title: Irreversible inhibition of trypsin by TLCK. A continuous method for kinetic study of irreversible enzymatic inhibitors in the presence of substrate.
    Author: Tudela J, Garcia-Canovas F, Garcia-Carmona F, Iborra JL, Lozano JA.
    Journal: Int J Biochem; 1986; 18(3):285-8. PubMed ID: 3956844.
    Abstract:
    This work presents a kinetic study on irreversible inhibition of trypsin by TLCK, using a new experimental approach. The method consists of the incubation of the enzyme with an irreversible inhibitor in the presence of a substrate which allows enzyme turnover as well as continuous measurement of the appearance of the product, a simultaneous change in the initial concentrations of the irreversible inhibitor and enzyme being undertaken, though a constant ratio between the latter, is maintained. This new approach enables the kinetic constants for TLCK, k2 and K1, to be determined.
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