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  • Title: Ultrathin-layer polyacrylamide gel isoelectric focusing for the identification of hemoglobin variants.
    Author: Budowle B, Eberhardt P.
    Journal: Hemoglobin; 1986; 10(2):161-72. PubMed ID: 3957695.
    Abstract:
    An ultrathin-layer polyacrylamide gel isoelectric focusing method for typing hemoglobin is described. This method, which uses Pharmalyte pH 6.7-7.7 and an electrode wick distance of 5.0 cm, clearly resolves the A, F, S, C and a number of rare variant allelic products. The technique is ideal for rapid screening of a large number of fresh blood and/or bloodstained samples.
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