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  • Title: Platelets, leucocytes and buffy layer vitamin C after surgery.
    Author: Vallance S.
    Journal: Hum Nutr Clin Nutr; 1986 Jan; 40(1):35-41. PubMed ID: 3957709.
    Abstract:
    The changes in buffy layer (BL) vitamin C concentration following surgical operations were studied in relation to changes in the leucocyte and platelet counts. It was found that the postoperative fall and subsequent changes in BL vitamin C concentration corresponded to changes in the platelet/leucocyte ratio (PLR) in whole blood and consequently in the buffy layer preparations from which vitamin C concentrations were measured. The results of the study showed that the fall in BL vitamin C measured following operations is an artifact of the method used rather than a demand for vitamin C. It highlights the importance of measuring the PLR, and suggests the need for a differential leucocyte count, when undertaking BL vitamin C analysis.
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