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  • Title: Biotinylated proteins as molecular weight standards on Western blots.
    Author: Della-Penna D, Christoffersen RE, Bennett AB.
    Journal: Anal Biochem; 1986 Feb 01; 152(2):329-32. PubMed ID: 3963367.
    Abstract:
    Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase complex and reaction with 4-chloro-1-naphthol and hydrogen peroxide. The biotinylated proteins are easy to prepare and are useful as molecular weight standards with most procedures employing immunodetection of proteins following transfer to nitrocellulose paper.
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