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  • Title: Tubulin subunit carboxyl termini determine polymerization efficiency.
    Author: Sackett DL, Bhattacharyya B, Wolff J.
    Journal: J Biol Chem; 1985 Jan 10; 260(1):43-5. PubMed ID: 3965457.
    Abstract:
    Cleavage of tubulin by subtilisin removes a small (Mr less than 2000) fragment from the C-terminal end of both alpha and beta subunits. The resulting protein is much reduced in negative charge. The cleaved, less acidic protein retains its competence to polymerize in a GTP-dependent and cold-, GDP-, and podophyllotoxin-sensitive manner and assembles into sheets or bundles of twisted filaments. The critical concentration for polymerization of the cleaved protein is about 50-fold lower than that for intact tubulin. It is proposed that the C termini of the subunits normally impede polymerization.
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