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  • Title: Purification of rabbit tumor necrosis factor.
    Author: Abe S, Gatanaga T, Yamazaki M, Soma G, Mizuno D.
    Journal: FEBS Lett; 1985 Jan 28; 180(2):203-6. PubMed ID: 3967764.
    Abstract:
    Rabbit tumor necrosis factor (TNF) was purified and shown by SDS-PAGE to be a single protein of 18 kDa. TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. By this procedure its yield was 22% and its specific activity was 2.4 X 10(7) U/mg protein. The sequence of the N-terminal 20 amino acids was determined.
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