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Title: Isolation of bovine eosinophils by density gradient centrifugation. Author: Chambers WH, Washburn SM, Campos M, Klesius PH. Journal: Am J Vet Res; 1985 Jan; 46(1):154-6. PubMed ID: 3970418. Abstract: A method for the isolation of an enriched population (greater than 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092. Viability was greater than or equal to 95%, and the isolated eosinophils were found to be functional in chemokinetic and antibody-dependent cellular-cytotoxicity assays. This method represents a simple, rapid method for obtaining bovine eosinophils that can be used in a variety of assays of eosinophil function.[Abstract] [Full Text] [Related] [New Search]