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  • Title: Isolation of bovine eosinophils by density gradient centrifugation.
    Author: Chambers WH, Washburn SM, Campos M, Klesius PH.
    Journal: Am J Vet Res; 1985 Jan; 46(1):154-6. PubMed ID: 3970418.
    Abstract:
    A method for the isolation of an enriched population (greater than 60%) of bovine eosinophils was developed, using density gradient centrifugation. Eosinophils were isolated from peripheral blood by centrifugation on a density gradient medium of sp gr 1.092. Viability was greater than or equal to 95%, and the isolated eosinophils were found to be functional in chemokinetic and antibody-dependent cellular-cytotoxicity assays. This method represents a simple, rapid method for obtaining bovine eosinophils that can be used in a variety of assays of eosinophil function.
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