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  • Title: Non-histone chromatin protein fractions associated with 'active' chromatin in embryonic chicken liver.
    Author: Siemieniako B, Rakowicz-Szulczyńska EM, Horst A.
    Journal: Mol Cell Biochem; 1985 Jan; 65(2):131-41. PubMed ID: 3982396.
    Abstract:
    Non-histone chromatin proteins synthesized during chicken embryonic liver development were labeled with [3H]tryptophan and [3H]methionine and characterized by electrophoresis. During embryonic development protein/DNA ratio in chromatin was low (1.30-1.62) but synthesis of non-histone protein was high. Especially one characteristic fraction K (MW 18 000), tightly bound with DNA was preferentially associated with DNAase II sensitive, active transcribed sequences. In 7-day old and adult chicken synthesis of all non-histone proteins was low, fraction K was absent or synthesized only in small amounts in association with non-active sequences, however protein/DNA ratio in chromatin was high (2.30-2.33).
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