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Title: Concomitant purification of three porcine heart mitochondrial enzymes: citrate synthase, aspartate aminotransferase, and malate dehydrogenase. Author: McEvily AJ, Flint AJ, Harrison JH. Journal: Anal Biochem; 1985 Jan; 144(1):159-64. PubMed ID: 3985311. Abstract: The mitochondrial enzymes citrate synthase, malate dehydrogenase, and aspartate aminotransferase were purified to homogeneity from porcine hearts by use of Bio-Rex 70, carboxymethylcellulose CM32, and Affi-Gel blue chromatography. This procedure provides relatively rapid, large-scale preparation of the three enzymes based on their differential binding to commercially available cation-exchange resins followed by a final affinity chromatography step.[Abstract] [Full Text] [Related] [New Search]